Explore the
Chimeric Antibody Development Platform
Chimeric antibodies are antibodies combining parental antibody variable regions with any acceptor constant region. Thanks to the domain organization of antibodies, chimerization is straightforward and does not alter antibody affinity or specificity. The combination of rodent variable domains with human constant domains is the most widespread, however, we can combine your variable domains with constant domains of diverse species.

Applications:

  • Therapeutic: Antibody chimerization is a preliminary step before antibody humanization in order to associate biological function to parental antibody and to decrease its immunogenicity. Alternatively, chimeric antibodies can be employed for therapeutic purposes in particular diseases (e.g. for immunocompromised patients) or under certain conditions (variable domain with high GI).
  • Diagnostic: Chimeric antibodies are the best alternative to serum, calibrators and positive controls in diagnostics assays. They are particularly suitable as they can be produced in unlimited quantities and homogeneous (preservation of antibody specificity and affinity.
BIOTEM chimerization platform combines parental variable domains to any constant domain (IgG1, IgG4, IgM etc.).

Parental Antibody Development

Depending on the stage of your project, BIOTEM proposes to take care of: Alternatively, chimerization can be implemented from any parental antibody sequences
  • BIOTEM’s added value
  • Quality validated antibodies
  • Various isotypes available
  • Small & large scale production
  • Parental antibody development and/or sequencing integrated if required

Chimeric Antibody Design

Production & Purification

Construction of chimeric antibody expression vectors

  • Chimeric antibody design:
    • Codon optimization for mammalian expression system
    • Choice of constant domain: species and isotype
  • Variable domain synthesis
  • Variable domains VH and VL cloning (2 expression plasmids)
  • Expression vector validation by their sequencing
Chimeric antibody production and purification

  • Transient transfection in CHO cells
  • Small and large scale production (up to several grams)
  • Protein A, G, A/G or L affinity chromatography
  • Productivity evaluation


Quality Control