Immunoassay development

The development of immunoassay for antigens detection, analysis and quantification require specific skills as well as a strong experience that BIOTEM brings to your project.

BIOTEM complies with the ISO9001:2008 quality standard and offers cutting edge services for the development and the validation of a large panel of assays (ELISA, Lateral flow, etc.)

BIOTEM Expertise
  • Several formats available
  • Sensibility (few ng/ml)
  • Qualitative or semi-quantitative
  • Recognised expertise
  • Validation / Batches delivery
in vitro Diagnostics
After the validation of a target biomarker, BIOTEM supports the development and validation of your immunoassay.

R & D
Immunoassays, and especially ELISA, are widely used tests that enable daily advances in science.


Quality controls
Immunoassays are often used as monitoring tools. Rapid tests offer a reliable answer to specific analyte detection requirements
Preliminary

A regular contact with our technical team provides the best conditions for BIOTEM to accompany and counsel you in your project development.

  • Custom approach
  • Format choice
  • Feasibility study and bibliography
  • Selection of test components
  • Validation of a development strategy
Validation

The assay development includes several validation steps that BIOTEM integrates in its process in order to meet your specifications.

  • Limit of detection / quantification range
  • Assay robustness and reproducibility (intra and inter test variation)
  • Specificity, cross reactivity, parallelism
  • Stability of test components, etc.
  • Parameter optimisation (i.e. reaction time, Km, sensibility)
Lateral flow

Lateral flow is an immunochromatographic assay developed initially for rapid medical diagnostics for home testing (pregnancy test, glycemia) and laboratory use.

BIOTEM has acquired a complete technical platform (BIODOT) which allows the development of all formats of Lateral flow assay.

Ask our scientific team for advice on development strategies. A technical and bibliographical feasibility study is performed free of charge in order to validate your project.

Human MAb

Human monoclonal antibody

Donor selection, PBMC separation, Activation, Serology, Optimised EBV immortalisation, Screening, Cloning, learn more...

Mouse MAb

Murine monoclonal
antibody development

Genetic immunisation, Subtractive, Fusion, R.A.D ® protocol, Screening, Confirmation, Cyclop Screening System, Cloning, Banking learn more...