Production, Purification &
Modification
Different methods of production and purification are recommended depending on the amount of antibodies required, the intended applications and the intrinsic properties of the hybridomas (productivity, stability, isotype...).
Whatever the specifications, BIOTEM can propose tailored solutions and its expertise to successfully complete each project.
In vitro
Production
In vivo
Production
(from nude and BALB/c mice)
The ascitic fluid production enables the obtention of high yields of antibody.
Purification
Depending on the physicochemicals characteristics of the antibody, the isotype and the origin (species, matrix, etc.), different purification strategies can be implemented (cf. table)
BIOTEM Expertise
Our know-how and several proprietary protocols enable us to complete sucessfully the most difficult purifications (IgM, double isotype...)
Some preliminary studies can be performed in order to determine the most optimal protocol(s) in terms of yield and purity.
Endotoxin-free batches
For each purification, BIOTEM uses validated and certified endotoxin-free material. In order to meet the requirements of European Pharmacopoeia, BIOTEM provides, upon request, purification on polymixin columns and delivers batches with conform standards of endotoxin level for your application.
Modifications
In order to provide you with the most efficient tool for your applications, BIOTEM proposes several antibody modifications, conjugations and fragmentations
.
BIOTEM Expertise
BIOTEM can assist you setting up your unique protocol, depending on your requirement, either using described techniques or combining strategies, etc.
A feasability study can be implemented to highlight the best strategy.
Immunoassay
development
ELISA, Lateral Flow
Immunoassays, Format choice (ELISA, Lateral flow, DIGFA, etc.) Detection / Quantification range, Reaction time, Km, Sensibility, BIODOT, Rapid test, learn more...
