Custom Polyclonal Antibody Production
Developing polyclonal antibodies is a convenient and versatile approach for the detection of target antigens. Obtaining a quality polyclonal serum that meets all specifications (application, specificity, cross-reaction, etc.) requires the implementation of a specific protocol.
BIOTEM offers customized protocols according to the client’s specifications. The company implements high quality production for a guaranteed result.
Polyclonal Antibody (pAb) – Definition & Applications
A polyclonal antibody (pAb) is a heterogeneous mixture of immunoglobulin molecules produced by multiple B-cell clones in response to an antigen. Unlike monoclonal antibodies (mAbs), which recognize a single epitope, polyclonal antibodies bind to multiple epitopes on the same antigen, making them highly sensitive and versatile for various biotechnological and biomedical applications.
Advantages of Using Polyclonal Antibodies
Polyclonal antibodies offer several advantages over monoclonal antibodies (mAb), making them valuable tools in biomedical research, diagnostics, and therapeutic development.
1. High Sensitivity and Strong Signal
- Polyclonal antibodies recognize multiple epitopes on the same antigen, leading to higher binding affinity.
- Enhanced signal amplification in immunoassays like ELISA, Western blot, and immunohistochemistry (IHC).
2. Broad Target Recognition
- Can bind to different antigenic variants and post-translationally modified proteins (PTMs).
- Useful in detecting low-abundance proteins and structural variations in target molecules.
3. Faster and Cost-Effective Production
- Compared to monoclonal antibodies, pAbs can be generated more quickly and at lower cost.
- Production in animals (e.g., rabbits, goats) is easier and scalable for large batches.
4. Greater Stability and Robustness
- Less sensitive to small antigen changes (mutations, conformational shifts).
- Work effectively in diverse conditions (e.g., harsh buffers, different pH levels).
5. Ideal for Immunotherapy Characterization
- Crucial for evaluating therapeutic antibodies, CAR-T cell therapies, and immune checkpoint inhibitors.
- Helps detect biomarkers and monitor immune responses in drug development.
Key Applications of Polyclonal Antibodies in Research & Industry:
- Biomedical Research – Protein detection, immune profiling, and cell signaling studies.
- Diagnostics – Infectious disease testing, biomarker identification, and serological assays.
- Biopharmaceutical Development – Drug target validation, quality control, and immunogenicity assessment.
BIOTEM does not claim any intellectual properties nor any other rights on the developed antibodies.
Build your own project:
- Custom Protocols (injections, bleeds, etc.)
- Several Species: Rabbits, rats, mice, goat, guinea pig, chicken, etc.
- Immune Response Monitoring
- Serum and/or Purified Antibody
Project
Specifications
1. Project Specifications
For each project, our team explores with the client the challenges of the project and defines together the specifications.
- The target of interest: membrane protein, peptide, small molecule, post-translational modifications, etc.
- Applications: ELISA, WB, FACS, IF, IHC, IP, HCP analysis (Host Cell Protein), functional tests, diagnostic application, etc.
- Specifications: Available material, specificity, deadlines, etc.
Considering these multiple parameters, BIOTEM proposes tailor-made strategies to meet the client's needs and guarantee the success of the project.
Immunogen
Preparation
2. Immunogen Preparation
Depending on the type of immunogen (cellular antigen, protein, peptide, nucleic acid, chemical molecule, etc.), BIOTEM offers specific design and preparation strategies.- Proteins: If the purified protein is not available, BIOTEM can assist the client in the production of recombinant proteins (transient transfection in CHO).
- Peptides: BIOTEM has the experience and know-how to optimally integrate all the parameters of immunogenic peptide design.
- Cellular antigens: Cells, viruses, bacteria, etc.
- Other: Chemical molecules (haptens), lipids, nucleic acids, etc.
Immunization
Several species
3. Immunization Several species BIOTEM has developed standardized protocols for rabbits that can be modified according to the client's specifications (duration of immunization, number and frequency of injections, co-immunization...). BIOTEM also offers a wide choice of animal species (rabbits, guinea pigs, goats, mice, rats, etc.). During the program, the client will have the opportunity to test the intermediate biological material in order to adjust the protocol if necessary.Purification
Antibody
4. Serum Purification
According to request, BIOTEM has several purification systems. Before sending the material, strict quality controls are carried out.
- Protein A / Protein G / Protein L Chromatography Purification
- Chromatography Purification against the antigen (protein / peptide)
- Differential Purification (phospho specific, other post-translational modification, etc.)
To access the document, please fill the form
