Antibody Characterization

1. Antibody Sequencing

BIOTEM offers services for sequencing variable and constant regions of antibodies from hybridomas or even purified antibodies ("de proteo" sequencing). Having the sequence of a monoclonal antibody allows:

• Hybridoma rescue
• Recombination and Redundancy analysis
• Antibody Engineering (Humanization, Affinity maturation, Antibody formats, etc. )

2. Affinity Determination

BIOTEM offers different strategies for affinity determination, including the "bio-layer interferometry" technique (BLItz® System, PALL FortéBio or the BiacoreTM). The determination of k_D , k_on, k_off is useful during an antibody development program to identify candidates with the best properties. This data is also necessary in order to best characterize a candidate antibody for patent application.

3. Cell Bank

• Master and Working Cell Bank
• CNCM Hybridoma bailment (Collection Nationale de Cultures de Microorganismes): Cell lines registration for regulatory purposes requires several procedures and validations. As an expert of hybridoma handling, BIOTEM supports its customers in the recording process with specific institutions
• Storage in liquid nitrogen
• Hybridoma monitoring contract : recurring thawing thaw, amplification and viability quantification
• Detection, removal of mycoplasma and Endotoxin-free treatment

4. Additional Services

Thanks to the know-how developed in immunotechnology since 1980 and the partnership with selected contractors, BIOTEM is able to propose a large spectrum of services enabling your objectives to be achieved.

• Stability study
• Epitope mapping
• Specific Conjugation, learn more
• Immunogenicity, Toxicity and Anatomopathology studies
• Isotyping
• Antibody Engineering
• Quality controls
• Custom studies
• Non-exhaustive list