Polyclonal antibodies

BIOTEM expertise
  • Host species
  • Immunogen
  • Method to assess the
    response
  • Immunisation protocols
  • Purification (relevance and
    considered method)

Immunogen Preparation

Peptide design

  • Homology
  • Hydrophobicity
  • Native protein investigation
  • Post-translational modification
  • Non-exhaustive list

Additional preparation

  • Preparation from SDS page
  • Plasmid injection
  • Protein injection
  • Preparation from bacteria lysate

Peptide synthesis

  • 70 to 99 % purity
  • from 1mg to >1g
  • Modification available: phosphorylation, nitration, cyclisation
    Complete list upon request

Conjugation

  • KLH, BSA, OVA
  • Carrier protein X for immunisation
  • Carrier protein Y pour ELISA titer
  • Antibody purification resin

Immunisations

Build you own project

Standard protocol proposed with NZW rabbit

Injections

D00: Immunisation 1 (ID with FCA)
D14: Immunisation 2 (SC with FIA)
D28: Immunisation 3 (SC with FIA)
D42: Immunisation 4 (SC with FIA)
D56: Immunisation 5 (SC with FIA)

Bleed

D00 : Pre-immune bleed (1 mL)
D49 : Bleed (20 to 25 mL)
D63 : Bleed (20 to 25 mL)
D77 : Bleed (20 to 25 mL)

- Several species available
(Wistar Rat, Hartley guinea pig, Balb/c mice, EOPS chicken, sheep, Goat)
- Feasible co-immunisation (multi-immunogen)
- Flexible ways and number of injections
- Number of bleed
- ELISA titer
- Protocol extension
- Flexible number of animal

- Final bleed: additional serum

Purifications

- Any volume
- Protein A / G / L affinity chromatography purifications
- Affinity chromatography against protein(s)
- Affinity chromatography against peptide(s)

- Phospho-specific purification
- Differential purifications

Quality controls

- Concentration determination by
spectrophotometric reading
- Purity control by polyacrylamide
gel eletrophoresis
Monoclonal
Murine hybridoma development

Genetic immunisation, Subtractive immunisation, Fusion, R.A.D ® protocol, Screening, Confirmations, Cyclop Screening System, Cloning, results commitments, learn more...