scFv antibody for your CAR-T cells

Main Components of a CAR-T cell:

• Surface Receptor against Tumor Antigen

This receptor is an ANTIBODY FRAGMENT that has been specifically developed against the marker of interest. Most of the time, the antibody fragment is a scFv (single chain fragment variable) which consists of variable regions of heavy (VH) and light (VL) chains associated with a flexible linker.

• Spacer/ Hinge Domain
• Transmembrane Domain
• Costimulatory Domain
• Activator Domain

Other applications for scFv

• Medical Field: Intrabody

Due to its small size (25kDa), scFv can be easily expressed in cells. Furthermore, the recombinant format allows for easy insertion of sequences to address the antibody to specific cellular compartments. Then, the scFv can neutralize/block and also monitor an intracellular marker, which raises the possibility of innovative therapies.

• Diagnostics

The size of the scFv and its expression as recombinant antibodies also allows the possibility of generating fusion proteins with different types of molecules (enzyme, fluorescent molecules, etc.). This makes the antibody fragment an excellent tool for immunoassay development.

BIOTEM’s Phage Display and Recombinant Antibody Production Platform

Learn more:

• Recombinant Antibody Development by Phage Display: https://www.biotem-antibody.com/custom-antibody-production/recombinant-monoclonal-antibody/
• Recombinant Antibody Production (transient transfection): https://www.biotem-antibody.com/custom-antibody-production/recombinant-antibody-production/

References:

[1]  Kochenderfer JN, Wilson WH, Janik JE, et al. Eradication of B-lineage cells and regression of lymphoma in a patient treated with autologous T cells genetically engineered to recognize CD19. Blood 2010 ; 116 : 4099–4102.

[2] https://www.fda.gov/news-events/press-announcements/fda-approval-brings-first-gene-therapy-united-states

By Jonathan MAYALI